MicroRNA files in the prevention of intestinal ischemia/reperfusion injury by hydrogen rich saline.
Weifeng Yao, Xiaoyu Lin, Xue Han, Lanfen Zeng, Anshun Guo, Yu Guan, Ziqing Hei, Jianpei Liu, Pinjie Huang · Bioscience reports · 2020
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Independent study record
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H2HUBB TAKEAWAY
In in vitro experiment, HRS treatment reduced miR-199a-3p level, increase IGF-1, PI3K and mTOR mRNA expression, restore IEC-6 cells viability, and this protective effects were reversed under miR-199a-3p mimics treatment. The authors concluded that miR-199a-3p may serve a key role in the anti-apoptotic mechanism of HRS that contributes to its protection of the intestine against I/R injury. These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.
What the Researchers Tested
Having recent highlight of microRNAs (miRNAs) in mediating intestinal I/R injury, the researchers hypothesized that HRS may protect intestine against I/R injury by regulating miRNAs.
Laboratory Model
Next, miR-199a-3p mimics or inhibitors were transfected into IEC-6 cells to explore the relationship between HRS treatment and miR-199a-3p.
What Molecular Hydrogen Changed
In in vitro experiment, HRS treatment reduced miR-199a-3p level, increase IGF-1, PI3K and mTOR mRNA expression, restore IEC-6 cells viability, and this protective effects were reversed under miR-199a-3p mimics treatment.
Authors’ Conclusion
These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.