Mitigation of Ischemia-Reperfusion Injury and Improvement in Overall Graft Viability by Hypothermic Pulsatile Perfusion with Molecular Hydrogen Is Associated with Trx-1/HO-1 Activation in a Non-Survival Ex Vivo Swine Model of Donation-After-Circulatory-Death Kidney Preservation and Transplantation.
George J Dugbartey, Cora England, Tamara S Ortas, Mahmoud Richard-Mohamed, Larry Jiang, Talal Shamma, Martin Igbokwe, Ali Bozaci, Juan Gonzalez Oyarzun, David Seok, Saeeda A Zainul, Lori Harrow, Monica Freeman, Renee Lindo-Anu, Aushanth Ruthirakanthan, Abdullah Alfaifi, John Wang, Patrick McLeod, Aaron Haig, Christopher Bonham, Alp Sener · International journal of molecular sciences · 2026
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In The researchers investigated the effect of H₂ in an ex vivo porcine model of DCD kidney transplantation, H₂ preserved renal architecture, evidenced by significantly reduced tubular necrosis and renal expression of damage markers, which corresponded with the downregulated renal expression of pro-inflammatory genes compared to the UW-only group (p < 0.05). The authors concluded that improvement in renal graft quality and function is associated with Trx-1/HO-1 activation, suggesting preliminary clinical trials in kidney transplantation. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in The researchers investigated the effect of H₂ in an ex vivo porcine model of DCD kidney transplantation. H₂ preserved renal architecture, evidenced by significantly reduced tubular necrosis and renal expression of damage markers, which corresponded with the downregulated renal expression of pro-inflammatory genes compared to the UW-only group (p < 0.05).
What the Researchers Studied
The researchers studied The researchers investigated the effect of H₂ in an ex vivo porcine model of DCD kidney transplantation. The study used a laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Left and right donor kidneys from 6 pigs were randomly assigned to either the UW-only group (control; n = 6) or the hydrogen-saturated University of Wisconsin (UW) solution (UW + H₂; 0.5 mM H₂ ) group ( n = 6), as illustrated in Figure 5 A. Prior to its addition to UW solution, H₂ was titrated to determine its concentration using methylene blue as an indicator. H₂ preserved renal architecture, evidenced by significantly reduced tubular necrosis and renal expression of damage markers, which corresponded with the downregulated renal expression of pro-inflammatory genes compared to the UW-only group (p < 0.05). The authors concluded that improvement in renal graft quality and function is associated with Trx-1/HO-1 activation, suggesting preliminary clinical trials in kidney transplantation.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was The researchers investigated the effect of H₂ in an ex vivo porcine model of DCD kidney transplantation. The study used a laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.