High-concentration hydrogen protects mouse heart against ischemia/reperfusion injury through activation of thePI3K/Akt1 pathway.
Ouyang Chen, Zhiyong Cao, He Li, Zhouheng Ye, Rongjia Zhang, Ning Zhang, Junlong Huang, Ting Zhang, Liping Wang, Ling Han, Wenwu Liu, Xuejun Sun · Scientific reports · 2017
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In C57BL/6 mice, the study showed for the first time that HCH after myocardial I/R injury ameliorated myocardial injury in a mouse I/R model (67% H₂ ) and alleviated H/R-induced injury in NMCs in vitro (75% H₂ ); these effects were attributed to activation of the PI3K/Akt1 pathway. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in C57BL/6 mice. The study showed for the first time that HCH after myocardial I/R injury ameliorated myocardial injury in a mouse I/R model (67% H₂ ) and alleviated H/R-induced injury in NMCs in vitro (75% H₂ ); these effects were attributed to activation of the PI3K/Akt1 pathway.
What the Researchers Studied
The researchers studied C57BL/6 mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 67% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 67% source-reported H₂ gas concentration; 0 mL/min H₂ flow. The study showed for the first time that HCH after myocardial I/R injury ameliorated myocardial injury in a mouse I/R model (67% H₂ ) and alleviated H/R-induced injury in NMCs in vitro (75% H₂ ); these effects were attributed to activation of the PI3K/Akt1 pathway. In the authors' pilot study, different duration of ischemia was induced in animals, and significant protective effects of hydrogen were observed after 4-h ischemia (short ischemia has limited infarct area and prolonged ischemia results in a high mortality).
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was C57BL/6 mice. The study used a preclinical animal experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 67% source-reported H₂ gas concentration.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.