Hydrogen alleviates mitochondrial dysfunction and organ damage via autophagy‑mediated NLRP3 inflammasome inactivation in sepsis.
Hongguang Chen, Xing Mao, Xiaoyin Meng, Yuan Li, Jingcheng Feng, Linlin Zhang, Yang Zhang, Yaoqi Wang, Yonghao Yu, Keliang Xie · International journal of molecular medicine · 2019
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In The cells were resuspended in DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin and streptomycin to adjust the cell density to 1.5×10 6 /ml, H₂ alleviated the mitochondrial dysfunction in macrophages induced by LPS and ATP, and improved lung, liver and kidney injury. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in The cells were resuspended in DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin and streptomycin to adjust the cell density to 1.5×10 6 /ml. H₂ alleviated the mitochondrial dysfunction in macrophages induced by LPS and ATP, and improved lung, liver and kidney injury.
What the Researchers Studied
The researchers studied The cells were resuspended in DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin and streptomycin to adjust the cell density to 1.5×10 6 /ml. The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
H₂ alleviated the mitochondrial dysfunction in macrophages induced by LPS and ATP, and improved lung, liver and kidney injury.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was The cells were resuspended in DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin and streptomycin to adjust the cell density to 1.5×10 6 /ml. The reported sample size was 60. The study used a in vitro cell-culture laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.