Molecular Hydrogen Modulates the Baroreflex Activity and Reduces the Vascular Adrenoreceptor Sensitivity to Phenylephrine and Lung Inflammation in Rats with Pulmonary Hypertension.
Marina Artemieva, Larisa Kozaeva, Tatyana Kuropatkina, Khaidar Gufranov, Dmitrii Atiakshin, Natalia Medvedeva, Oleg Medvedev · Biomedicines · 2026
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In male Wistar rats, experiments on isolated aortic preparations from MCT rats showed that the addition of H₂ to the perfusion medium resulted in a 30% reduction in the maximal response to PE compared with the MCT group without hydrogen (p < 0.01), and the potency of PE (EC50) decreased threefold (p < 0.05). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how hydrogen-containing cold-water immersion affected the outcomes measured in male Wistar rats. Experiments on isolated aortic preparations from MCT rats showed that the addition of H₂ to the perfusion medium resulted in a 30% reduction in the maximal response to PE compared with the MCT group without hydrogen (p < 0.01), and the potency of PE (EC50) decreased threefold (p < 0.05).
What the Researchers Studied
The researchers studied male Wistar rats. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 4% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 4% source-reported H₂ gas concentration. Experiments on isolated aortic preparations from MCT rats showed that the addition of H₂ to the perfusion medium resulted in a 30% reduction in the maximal response to PE compared with the MCT group without hydrogen (p < 0.01), and the potency of PE (EC50) decreased threefold (p < 0.05). The authors concluded that the use of intermittent H₂ inhalation in the authors' work imitating the protocol of human studies revealed their moderate effect on the state of the cardiovascular system and lung tissue under MCT-PH. /Objectives: Molecular hydrogen (H₂), a natural antioxidant, can selectively reduce hydroxyl radicals and peroxynitrite without affecting signaling molecules such as H₂O₂ and NO.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was male Wistar rats. The study used a preclinical animal experiment. The hydrogen delivery method was hydrogen-containing cold-water immersion. The source-reported hydrogen concentration was 4% source-reported H₂ gas concentration.
Limitations and Safety
Reported limitations: Although a non-significant trend toward modulating endothelium-dependent relaxation was observed in the MCT-H₂ group, this interpretation is limited by the lower baseline of PE-induced precontraction in this group, a factor known to influence the amplitude of the relaxant response.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.