Molecular hydrogen reduces LPS-induced neuroinflammation and promotes recovery from sickness behaviour in mice.
Stefan Spulber, Karin Edoff, Lie Hong, Shinkatsu Morisawa, Sanetaka Shirahata, Sandra Ceccatelli · PloS one · 2012
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Independent study record
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H2HUBB TAKEAWAY
In mice, the effect of molecular hydrogen in drinking water at the timepoints corresponding to the recovery from sickness syndrome (24–48 h) is characterized by a lower upregulation of proinflammatory cytokines (IL-1β and IL-6), an earlier upregulation of BDNF, and an upregulation of IL-10, HO-1, and Nrf2 48 h after LPS administration only in H-ERW-treated mice. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in mice. The effect of molecular hydrogen in drinking water at the timepoints corresponding to the recovery from sickness syndrome (24–48 h) is characterized by a lower upregulation of proinflammatory cytokines (IL-1β and IL-6), an earlier upregulation of BDNF, and an upregulation of IL-10, HO-1, and Nrf2 48 h after LPS administration only in H-ERW-treated mice.
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
The effect of molecular hydrogen in drinking water at the timepoints corresponding to the recovery from sickness syndrome (24–48 h) is characterized by a lower upregulation of proinflammatory cytokines (IL-1β and IL-6), an earlier upregulation of BDNF, and an upregulation of IL-10, HO-1, and Nrf2 48 h after LPS administration only in H-ERW-treated mice. The authors concluded that consistently, molecular hydrogen modulates the activation and gene expression in a similar fashion in immortalized murine microglia (BV-2 cell line), suggesting that the effects observed in vivo may involve the modulation of microglial activation. Taken together, the data point to the regulation of cytokine expression being an additional critical mechanism underlying the beneficial effects of molecular hydrogen. In summary, molecular hydrogen induces a more robust induction of proinflammatory (TNF-α, IL-1β, IL-6) and antiinflammatory (IL-10) cytokines in the acute phase (2–4 h), followed by a faster decay in expression at later timepoints (24 h).
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.