ability with high mortality. Hydrogen-rich saline, which has therapeutic anti-inflammatory and anti-apoptotic activity, can attenuate pulmonary edema in sepsis-related lung injury. However, the mechanisms of the protective effect are
Kai Wang1*, Xiao Song1*, Shaoxia Duan2*, Wei Fang3, Xiang Huan1, Yu Cao4, Jiajia Tang2, Liwei Wang1 · 2017
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In male Sprague-Dawley rats, pretreatment with hydrogen-rich saline significantly alleviated pulmonary edema and attenuated the deterioration of claudin-5 protein induced by LPS in rat lung. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in male Sprague-Dawley rats. Pretreatment with hydrogen-rich saline significantly alleviated pulmonary edema and attenuated the deterioration of claudin-5 protein induced by LPS in rat lung.
What the Researchers Studied
The researchers studied male Sprague-Dawley rats. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Sixty male Sprague-Dawley rats, which were randomly divided into six groups (n = 10, in each group): control group, LPS group, LPS+H₂ group, H₂ group, LPS+H₂+LY294002 group and LPS+LY294002 group, received intratracheal administration of LPS followed by intraperitoneal injection of hydrogen-rich saline or intravenous injec- tion of PI3K inhibitor LY294002. Pretreatment with hydrogen-rich saline significantly alleviated pulmonary edema and attenuated the deterioration of claudin-5 protein induced by LPS in rat lung. The authors concluded that hydrogen-rich saline ameliorates LPS-induced ALI through reducing disruption of claudin-5 protein, which may be associated with the enhanced ac- tivation of the PI3K/Akt pathway. Hydrogen-rich saline enhanced LPS-induced activation of the PI3K/Akt pathway, which is associated with the regulation of claudin-5 expression.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was male Sprague-Dawley rats. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base. The original scholarly source is available at https://ijcem.com/files/ijcem0051149.pdf.