Neuroprotective effect of hydrogen-rich saline against neurologic damage and apoptosis in early brain injury following subarachnoid hemorrhage: possible role of the Akt/GSK3β signaling pathway.
Yuan Hong, AnWen Shao, Jianfeng Wang, Sheng Chen, HaiJian Wu, Devin W McBride, Qun Wu, XueJun Sun, JianMin Zhang · PloS one · 2014
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In Sprague-Dawley rats, bcl-2 was significantly increased while Bax and cleaved caspase-3 was decreased by HS treatment. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in Sprague-Dawley rats. Bcl-2 was significantly increased while Bax and cleaved caspase-3 was decreased by HS treatment.
What the Researchers Studied
The researchers studied Sprague-Dawley rats. The study used a preclinical animal experiment.
What Effects Did Molecular Hydrogen Have?
Sprague-Dawley rats (n = 85) were randomly divided into the following groups: sham group (n = 17), SAH + saline group (n = 17), SAH + hydrogen-rich saline (HS) group (n = 17) and SAH + HS + Ly294002 (n = 17) group. Bcl-2 was significantly increased while Bax and cleaved caspase-3 was decreased by HS treatment. The authors concluded that hS could attenuate neuronal apoptosis in EBI and improve the neurofunctional outcome after SAH, partially via the Akt/GSK3β pathway. However, the molecular mechanism underlying hydrogen-mediated anti-apoptotic effects in SAH has not been elucidated. In the present study, the researchers aimed to evaluate whether hydrogen alleviates EBI after SAH, specifically neuronal apoptosis, partially via the Akt/GSK3β signaling pathway.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was Sprague-Dawley rats. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.