Pharmacokinetics of hydrogen administered intraperitoneally as hydrogen-rich saline and its effect on ischemic neuronal cell death in the brain in gerbils.
Momoko Hirano, Kazuhisa Sugai, Masahiko Fujisawa, Eiji Kobayashi, Yoshinori Katsumata, Yoji Hakamata, Motoaki Sano · PloS one · 2022
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In a laboratory model, on day 3 of ischemia/reperfusion, the number of neurons undergoing apoptosis in the cortex was significantly lower in the H₂-rich saline group than in the saline group, and on day 7, the number of viable neurons in the hippocampus and cerebral cortex was significantly higher in the H₂-rich saline group. The authors concluded that these results indicate that intraperitoneal administration of H₂-rich saline significantly suppresses neuronal cell death after cerebral ischemia, even though H₂ hardly reaches the brain. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen was evaluated in this publication and summarizes the source-grounded findings below. On day 3 of ischemia/reperfusion, the number of neurons undergoing apoptosis in the cortex was significantly lower in the H₂-rich saline group than in the saline group, and on day 7, the number of viable neurons in the hippocampus and cerebral cortex was significantly higher in the H₂-rich saline group.
What the Researchers Studied
The study used a in vitro cell-culture laboratory experiment.
What Effects Did Molecular Hydrogen Have?
Intraperitoneal administration of hydrogen (H₂)-containing saline inhibited neuronal cell death in ischemic stroke in a number of animal models, but it is unknown whether H₂ is absorbed from the abdominal cavity into the blood and reaches the brain. In this study, the researchers investigated whether intraperitoneal administration of saline containing H₂ inhibits neuronal cell death caused by cerebral ischemia and measured the concentration of H₂ in the carotid artery and inferior vena cava (IVC). a single dose of saline or H₂-rich saline was administered intraperitoneally, and blood H₂ levels in the carotid artery and IVC were measured. On day 3 of ischemia/reperfusion, the number of neurons undergoing apoptosis in the cortex was significantly lower in the H₂-rich saline group than in the saline group, and on day 7, the number of viable neurons in the hippocampus and cerebral cortex was significantly higher in the H₂-rich saline group. The authors concluded that these results indicate that intraperitoneal administration of H₂-rich saline significantly suppresses neuronal cell death after cerebral ischemia, even though H₂ hardly reaches the brain.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The study used a in vitro cell-culture laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.