Protective effects of dissolved molecular hydrogen against hydrogen peroxide-, hydroperoxide-, and glyoxal-induced injuries to human skin keratinocytes.
Yasukazu Saitoh, Yuuki Yamaguchi, Yuhei Okada · Molecular and cellular biochemistry · 2021
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Independent study record
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H2HUBB TAKEAWAY
The results also revealed that H₂ suppressed H₂O₂-induced increases in intracellular peroxide and H₂O₂ levels, and suppressed the progression of lipid peroxidation. The authors concluded that taken together, the results demonstrate that H₂ can exert protective effects against oxidative stress-, lipid peroxidation-, and carbonyl stress-induced cellular injuries in human keratinocytes, partly mediated via suppression of intracellular oxidative stress and peroxide generation. These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.
What the Researchers Tested
In vitro cell-culture laboratory experiment.
Laboratory Model
The purpose of this study was to examine the protective effect of H₂ against ultraviolet (UV) irradiation-related stress injury in human epidermal HaCaT cells.
How Molecular Hydrogen Was Used
Molecular hydrogen (H₂) is recognized as a gaseous antioxidant, and it is expected to ameliorate various disorders related to oxidative stress and inflammation. Therefore, the purpose of this study was to examine the protective effect of H₂ against ultraviolet (UV) irradiation-related stress injury in human epidermal HaCaT cells. The researchers investigated the effects of H₂ against three types of UV-derived oxidative stress using human skin keratinocytes: hydrogen peroxide (H₂O₂)-induced oxidative stress, tert-butyl hydroperoxide (t-BuOOH)-induced lipid peroxidation stress, and glyoxal-induced carbonyl stress.
What Molecular Hydrogen Changed
The results also revealed that H₂ suppressed H₂O₂-induced increases in intracellular peroxide and H₂O₂ levels, and suppressed the progression of lipid peroxidation.
Proposed Mechanism
Molecular hydrogen (H₂) is recognized as a gaseous antioxidant, and it is expected to ameliorate various disorders related to oxidative stress and inflammation. The researchers investigated the effects of H₂ against three types of UV-derived oxidative stress using human skin keratinocytes: hydrogen peroxide (H₂O₂)-induced oxidative stress, tert-butyl hydroperoxide (t-BuOOH)-induced lipid peroxidation stress, and glyoxal-induced carbonyl stress.
Authors’ Conclusion
These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.