Protective effects of dissolved molecular hydrogen against hydrogen peroxide-, hydroperoxide-, and glyoxal-induced injuries to human skin keratinocytes.
Yasukazu Saitoh, Yuuki Yamaguchi, Yuhei Okada · Molecular and cellular biochemistry · 2021
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Independent study record
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H2HUBB TAKEAWAY
The results also revealed that H₂ suppressed H₂O₂-induced increases in intracellular peroxide and H₂O₂ levels, and suppressed the progression of lipid peroxidation. The authors concluded that taken together, the results demonstrate that H₂ can exert protective effects against oxidative stress-, lipid peroxidation-, and carbonyl stress-induced cellular injuries in human keratinocytes, partly mediated via suppression of intracellular oxidative stress and peroxide generation. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in Therefore, the purpose of this study was to examine the protective effect of H₂ against ultraviolet (UV) irradiation-related stress injury in human epidermal HaCaT cells. The results also revealed that H₂ suppressed H₂O₂-induced increases in intracellular peroxide and H₂O₂ levels, and suppressed the progression of lipid peroxidation.
What the Researchers Studied
The researchers studied Therefore, the purpose of this study was to examine the protective effect of H₂ against ultraviolet (UV) irradiation-related stress injury in human epidermal HaCaT cells. The study used a in vitro cell-culture laboratory experiment.
What Effects Did Molecular Hydrogen Have?
Molecular hydrogen (H₂) is recognized as a gaseous antioxidant, and it is expected to ameliorate various disorders related to oxidative stress and inflammation. the purpose of this study was to examine the protective effect of H₂ against ultraviolet (UV) irradiation-related stress injury in human epidermal HaCaT cells. The researchers investigated the effects of H₂ against three types of UV-derived oxidative stress using human skin keratinocytes: hydrogen peroxide (H₂O₂)-induced oxidative stress, tert-butyl hydroperoxide (t-BuOOH)-induced lipid peroxidation stress, and glyoxal-induced carbonyl stress. The results also revealed that H₂ suppressed H₂O₂-induced increases in intracellular peroxide and H₂O₂ levels, and suppressed the progression of lipid peroxidation. The authors concluded that taken together, the results demonstrate that H₂ can exert protective effects against oxidative stress-, lipid peroxidation-, and carbonyl stress-induced cellular injuries in human keratinocytes, partly mediated via suppression of intracellular oxidative stress and peroxide generation.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was Therefore, the purpose of this study was to examine the protective effect of H₂ against ultraviolet (UV) irradiation-related stress injury in human epidermal HaCaT cells. The study used a in vitro cell-culture laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.