Protective effects of molecular hydrogen on steroid-induced osteonecrosis in rabbits via reducing oxidative stress and apoptosis.
Jia Li, Zhaogang Ge, Lihong Fan, Kunzheng Wang · BMC musculoskeletal disorders · 2017
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Independent study record
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H2HUBB TAKEAWAY
In rabbits, the incidence of steroid-induced ON was significantly lower in hydrogen group (28.6%) than that in model group (68.0%). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in rabbits. The incidence of steroid-induced ON was significantly lower in hydrogen group (28.6%) than that in model group (68.0%).
What the Researchers Studied
The researchers studied rabbits. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
To MPSL, the hydrogen group also received intraperitoneal injections of molecular hydrogen at 10 ml/kg body weight for seven consecutive days starting from the day of MPSL administration, which was based on the period of greatest susceptibility to steroid-induced oxidative injury suggested by Ichiseki et al. [ 11, 28 ]. Briefly, sections were immersed in 3% hydrogen peroxide PBS for 10 min, rinsed several times in PBS, pretreated with 10% goat normal antiserum(Vector, Burlingame, California, USA) for 30 min at room temperature. The incidence of steroid-induced ON was significantly lower in hydrogen group (28.6%) than that in model group (68.0%). The authors concluded that these results suggested that molecular hydrogen prevents steroid-induced osteonecrosis in rabbits by suppressing oxidative injury, vascular injury and apoptosis. were attenuated in the hydrogen group compared with those in the model group in vivo.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was rabbits. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.