Protective Role of Hydrogen Gas on Oxidative Damage and Apoptosis in Intestinal Porcine Epithelial Cells (IPEC-J2) Induced by Deoxynivalenol: A Preliminary Study.
Xu Ji, Weijiang Zheng, Wen Yao · Toxins · 2019
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Independent study record
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H2HUBB TAKEAWAY
In In conclusion, H₂ could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro, however, H₂ efficiently reduced the LDH release ( p < 0.05) and increased cell viability ( p < 0.05) in the DON + H₂ group, suggesting that H₂ significantly alleviated DON-induced IPEC-J2 cell growth inhibition. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in In conclusion, H₂ could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro. However, H₂ efficiently reduced the LDH release ( p < 0.05) and increased cell viability ( p < 0.05) in the DON + H₂ group, suggesting that H₂ significantly alleviated DON-induced IPEC-J2 cell growth inhibition.
What the Researchers Studied
The researchers studied In conclusion, H₂ could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro. The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
The preparation and storage of H₂ -saturated medium were according to previous studies with some modifications [ 33 ]. Hydrogen was obtained from a hydrogen gas tank (≥99.999% purity) and then it was dissolved in serum-free DMEM/F12 in a Pytex bottle for 30 min under 0.2 MPa pressure to get a supersaturated H₂ concentration (≥0.6 mM). Subsequently, 10% FBS was added into the H₂ -saturated medium. However, H₂ efficiently reduced the LDH release ( p < 0.05) and increased cell viability ( p < 0.05) in the DON + H₂ group, suggesting that H₂ significantly alleviated DON-induced IPEC-J2 cell growth inhibition. The authors concluded that in conclusion, H₂ could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro. To explore the protective role of hydrogen gas (H₂) on oxidative damage and apoptosis in intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol (DON), cells were assigned to four treatment groups, including control, 5 μM DON, H₂-saturated medium, and 5 μM DON + H₂-saturated medium treatments. The present results indicate that hydrogen decreased the levels of •OH, ONOO −, and MDA, as well as increased the gene expression and activities of antioxidative enzymes in IPEC-J2 cells stimulated by DON.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was In conclusion, H₂ could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro. The study used a in vitro cell-culture laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.