Protective Role of Hydrogen Gas on Oxidative Damage and Apoptosis in Intestinal Porcine Epithelial Cells (IPEC-J2) Induced by Deoxynivalenol: A Preliminary Study.
Xu Ji, Weijiang Zheng, Wen Yao · Toxins · 2019
Research-use notice
Independent study record
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H2HUBB TAKEAWAY
In In conclusion, H₂ could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro, however, the H₂-saturated medium significantly improved cell growth status and reversed the change of redox states and expression of genes and proteins related to apoptosis induced by DON in IPEC-J2 cells. The authors concluded that in conclusion, H₂ could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro. These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.
What the Researchers Tested
In vitro cell-culture laboratory experiment.
Laboratory Model
In conclusion, H₂ could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro.
How Molecular Hydrogen Was Used
In conclusion, H₂ could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro.
What Molecular Hydrogen Changed
However, the H₂-saturated medium significantly improved cell growth status and reversed the change of redox states and expression of genes and proteins related to apoptosis induced by DON in IPEC-J2 cells.
Proposed Mechanism
To explore the protective role of hydrogen gas (H₂) on oxidative damage and apoptosis in intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol (DON), cells were assigned to four treatment groups, including control, 5 μM DON, H₂-saturated medium, and 5 μM DON + H₂-saturated medium treatments.
Authors’ Conclusion
These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.