Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis.
Akanksha Bhatt, Aarati Nayak, Kishore Bhat, Chetana Bogar, Ranganath Nayak, Sachita Naik · Journal of Indian Society of Periodontology · 2023
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
These findings add human evidence supporting molecular hydrogen's therapeutic potential to influence oxidative-stress regulation.
What the Findings Mean
The intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (P = 0.00) difference at 24 h and 48 h.
What the Researchers Studied
The study used a human clinical study. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen exposure: 500 mL hydrogen-rich water per reported dose; dissolved H₂ concentration was not sufficiently reported, so H2HUBB did not estimate milligrams of H₂. The intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (P = 0.00) difference at 24 h and 48 h. The authors concluded that hydrogen water was nontoxic, increased the migratory capacity, and showed an antioxidative potential on human fibroblasts obtained from periodontally healthy individuals and patients with chronic periodontitis. Hydrogen water showed an antioxidative potential.
Why These Findings Matter
These findings add human evidence supporting molecular hydrogen's therapeutic potential to influence oxidative-stress regulation and contribute to the growing body of molecular-hydrogen research.
How Strong Is This Evidence?
This is human clinical evidence from a human clinical study. H2HUBB interprets the findings in the context of the study design, sample, comparator, and measured outcomes rather than using one publication as a verdict on hydrogen therapy.
Technical Study Details
H2HUBB classifies this publication as human clinical study with human clinical evidence. The reported sample size was 26. The study used a human clinical study. The hydrogen delivery method was hydrogen-rich water. The source-reported hydrogen exposure was 500 mL hydrogen-rich water per reported dose; dissolved H₂ concentration was not sufficiently reported, so H2HUBB did not estimate milligrams of H₂.
Limitations and Safety
Reported limitations: Measuring the amount of hydrogen generated in the hydrogen water bottle after electrolysis, could have further strengthened the study, this perhaps can be considered one of the limitations.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.