Hydrogen Research Study
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Human clinical evidenceHuman Clinical StudyOther or not reported

Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis.

Akanksha Bhatt, Aarati Nayak, Kishore Bhat, Chetana Bogar, Ranganath Nayak, Sachita Naik · Journal of Indian Society of Periodontology · 2023

Research-use notice

Independent study record

Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.

H2HUBB Research Library branded molecular hydrogen research image
Primary topic Dental and Oral Health
Evidence type Human clinical evidence
Publication type Human Clinical Study
Hydrogen method Other or not reported

H2HUBB TAKEAWAY

In 26 participants, the intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (P = 0.00) difference at 24 h and 48 h. These findings add human evidence supporting molecular hydrogen's therapeutic potential to influence oxidative-stress regulation.

What the Findings Mean

H2HUBB reviewed how molecular hydrogen affected the outcomes measured in 26 participants. The intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (P = 0.00) difference at 24 h and 48 h.

What the Researchers Studied

The researchers studied 26 participants. The study used a human clinical study.

What Effects Did Molecular Hydrogen Have?

The effect of hydrogen water on cell viability was checked by neutral red assay, while the migration potential was assessed by transwell migration assay. The antioxidative potential of hydrogen water was evaluated by CUPRAC assay. The intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (P = 0.00) difference at 24 h and 48 h. The authors concluded that hydrogen water was nontoxic, increased the migratory capacity, and showed an antioxidative potential on human fibroblasts obtained from periodontally healthy individuals and patients with chronic periodontitis. Hydrogen water showed an antioxidative potential.

Why These Findings Matter

These findings add human evidence supporting molecular hydrogen's therapeutic potential to influence oxidative-stress regulation and contribute to the growing body of molecular-hydrogen research.

How Strong Is This Evidence?

This is human clinical evidence from a human clinical study. H2HUBB interprets the findings in the context of the study design, sample, comparator, and measured outcomes rather than using one publication as a verdict on hydrogen therapy.

Technical Study Details

H2HUBB classifies this publication as human clinical study with human clinical evidence. The research population or model was 26 participants. The reported sample size was 26. The study used a human clinical study.

Limitations and Safety

No separate limitations or safety findings were identified in the source text available to H2HUBB.

Original Study and H2HUBB Research Context

H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.