Effects of Hydrogen-Rich Water on Growth, Redox Homeostasis and Hormonal, Histological and Immune Systems in Rats Exposed to High Cage Density Stress.
Buket Boğa Kuru, Mustafa Makav, Mushap Kuru, Şükran Yediel Aras, Ebru Karadağ Sarı, Menekşe Bulut, Duried Alwazeer, Fikret Bektaşoğlu, Mükremin Ölmez, Turgut Kırmızıbayrak, Tyler W LeBaron · Veterinary medicine and science · 2025
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In rats, oxidant status in biochemical analyses decreased in the stress + HRW group compared to the stress group. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in rats. Oxidant status in biochemical analyses decreased in the stress + HRW group compared to the stress group.
What the Researchers Studied
The researchers studied rats. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen exposure: 2 L hydrogen-rich water per reported dose; dissolved H₂ concentration was not sufficiently reported, so H2HUBB did not estimate milligrams of H₂; reported treatment duration: 3 months. Oxidant status in biochemical analyses decreased in the stress + HRW group compared to the stress group. The authors concluded that the stress-related physiological parameters were reduced in the HRW + stress group compared to the stress group. This study investigated the impact of drinking hydrogen-rich water (HRW) on growth performance, organ weights, thiol/disulphide homeostasis, oxidative status and some hormonal, histopathological and immunohistochemical changes in rats fed in a restricted housing environment. The high levels of thiols in the HRW‐treated groups, especially in the stress + HRW group, indicate that the molecular hydrogen reduces oxidative stress and decreases the consumption of thiols in stress conditions.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was rats. The reported sample size was 32. The study used a preclinical animal experiment. The hydrogen delivery method was hydrogen-rich water. The source-reported hydrogen exposure was 2 L hydrogen-rich water per reported dose; dissolved H₂ concentration was not sufficiently reported, so H2HUBB did not estimate milligrams of H₂. The reported treatment duration was 3 months.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.