Evaluation of the therapeutic effects of nebulized inhalation of hydrogen-rich water on primary blast lung injury in C57BL/6 mice.
Yirui Qu, Qiaoling Chen, Jiake Chai, Fangchao Hu, Tian Liu, Xiangyu Liu, Hongjie Duan, Yunfei Chi · Surgery · 2025
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In C57BL/6 mice, levels of inflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor α) and oxidative stress markers (ie, malondialdehyde) were significantly lower, whereas antioxidant enzyme (total superoxide dismutase) activity was higher in the nebulized hydrogen-rich water treatment group compared with the blast injury control group (P <.01). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in C57BL/6 mice. Levels of inflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor α) and oxidative stress markers (ie, malondialdehyde) were significantly lower, whereas antioxidant enzyme (total superoxide dismutase) activity was higher in the nebulized hydrogen-rich water treatment group compared with the blast injury control group (P <.01).
What the Researchers Studied
The researchers studied C57BL/6 mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Mice were exposed to a blast overpressure of 266 ± 9.156 kPA and treated with either nebulized hydrogen-rich water or sterile injection water immediately postinjury. Levels of inflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor α) and oxidative stress markers (ie, malondialdehyde) were significantly lower, whereas antioxidant enzyme (total superoxide dismutase) activity was higher in the nebulized hydrogen-rich water treatment group compared with the blast injury control group (P <.01). The authors concluded that hydrogen's antioxidant and anti-inflammatory properties play a crucial role in mitigating lung damage and improving respiratory function postinjury.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was C57BL/6 mice. The study used a preclinical animal experiment. The hydrogen delivery method was hydrogen-rich water.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.