Hydrogen gas protects against serum and glucose deprivation‑induced myocardial injury in H9c2 cells through activation of the NF‑E2‑related factor 2/heme oxygenase 1 signaling pathway.
Qiang Xie, Xue-Xiang Li, Peng Zhang, Jin-Cao Li, Ying Cheng, Yan-Ling Feng, Bing-Sheng Huang, Yu-Feng Zhuo, Guo-Hua Xu · Molecular medicine reports · 2014
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In a laboratory model, when the cells were exposed to SGD, levels of intracellular ·OH free radicals were markedly increased; this was mitigated by H₂ gas‑rich medium. The authors concluded that in conclusion, H₂ gas protected cardiomyocytes from ischemia‑induced myocardial injury through elimination of ·OH free radicals and also through activation of the Nrf2/HO‑1 signaling pathway. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas was evaluated in this publication and summarizes the source-grounded findings below. When the cells were exposed to SGD, levels of intracellular ·OH free radicals were markedly increased; this was mitigated by H₂ gas‑rich medium.
What the Researchers Studied
The study used a in vitro cell-culture laboratory experiment.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 99.999% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 99.999% source-reported H₂ gas concentration. When the cells were exposed to SGD, levels of intracellular ·OH free radicals were markedly increased; this was mitigated by H₂ gas‑rich medium. The authors concluded that in conclusion, H₂ gas protected cardiomyocytes from ischemia‑induced myocardial injury through elimination of ·OH free radicals and also through activation of the Nrf2/HO‑1 signaling pathway. Hydrogen (H₂) gas, as a water‑ and lipid‑soluble small molecule, is not only able to selectively eliminate hydroxyl (·OH) free radicals, but also to enhance endogenous antioxidative defense systems in rat lungs and arabidopsis plants. Oxidative stress is characterized by marked increases in the production of reactive oxygen species (ROS), including the superoxide anion (O₂ •-) and the hydroxyl radical (·OH), as well as non -radical molecules, including hydrogen peroxide (H₂O₂) and singlet oxygen (1O2) (3,4). The authors linked these findings to activation of the NF‑E2‑related factor 2/heme oxygenase 1 signaling pathway.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The study used a in vitro cell-culture laboratory experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 99.999% source-reported H₂ gas concentration.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base. The original scholarly source is available at https://www.spandidos-publications.com/10.3892/mmr.2014.2283/download.