Hydrogen Research Study
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Human clinical evidenceHuman Clinical StudyOther or not reported

Hydrogen Gas Inhalation Improved Intestinal Microbiota in Ulcerative Colitis: A Randomised Double-Blind Placebo-Controlled Trial.

Takafumi Maruyama, Dai Ishikawa, Rina Kurokawa, Hiroaki Masuoka, Kei Nomura, Mayuko Haraikawa, Masayuki Orikasa, Rina Odakura, Masao Koma, Masashi Omori, Hirotaka Ishino, Kentaro Ito, Tomoyoshi Shibuya, Wataru Suda, Akihito Nagahara · Biomedicines · 2025

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Independent study record

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H2HUBB Research Library branded molecular hydrogen research image
Primary topic Gastrointestinal Health
Evidence type Human clinical evidence
Publication type Human Clinical Study
Hydrogen method Other or not reported

How Molecular Hydrogen Was Used

In this randomised, double-blind, placebo-controlled trial, 10 active patients with ulcerative colitis (aged ≥20 years; Lichtiger's clinical activity index, 3-10; and Mayo endoscopic subscores ≥1) participated, and they were assigned to either a hydrogen or air inhalation group (hydrogen and placebo groups, respectively).

What the Researchers Found

The hydrogen group showed higher α-diversity (p = 0.19), and the variation in β-diversity was markedly different, compared to the placebo group, in intestinal microbiota analysis (p = 0.02). Functional gene analysis revealed 115 significant genetic changes in the hydrogen group following treatment.

H₂ Mechanisms / Biological Findings

The source material reviewed did not establish a specific molecular hydrogen mechanism for the reported findings.

Authors’ Conclusion

The authors concluded that hydrogen inhalation appeared to improve intestinal microbiota diversity; however, no clear therapeutic effect on ulcerative colitis was observed. Further studies are needed, and hydrogen inhalation may possibly lead to a logical solution combined with microbiome therapy, such as faecal microbiota transplantation, with fewer adverse events.

H2HUBB TAKEAWAY

The hydrogen group showed higher α-diversity, and the variation in β-diversity was markedly different, compared to the placebo group, in intestinal microbiota analysis.

What the Researchers Studied

The study examined 10 patients with ulcerative colitis (aged ≥20 years.