HYDROGEN-RICH MEDIUM AMELIORATES LIPOPOLYSACCHARIDE-INDUCED BARRIER DYSFUNCTION VIA RHOA-MDIA1 SIGNALING IN CACO-2 CELLS.
Tao Yang, Lu Wang, Ruiqiang Sun, Hongguang Chen, Hongtao Zhang, Yang Yu, Yanyan Wang, Guolin Wang, Yonghao Yu, Keliang Xie · Shock (Augusta, Ga.) · 2016
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In Caco-2 cells were exposed to different concentrations of LPS (1 μg/mL-1 mg/mL), lPS (100 μg/mL) decreased TER and increased fluorescein-isothiocyanate-dextran flux, which were alleviated by H₂-rich medium. The authors concluded that H₂ improves LPS-induced hyperpermeability of the intestinal barrier and disruptions of TJ and AJ by moderating RhoA-mDia1 signaling. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in Caco-2 cells were exposed to different concentrations of LPS (1 μg/mL-1 mg/mL). LPS (100 μg/mL) decreased TER and increased fluorescein-isothiocyanate-dextran flux, which were alleviated by H₂-rich medium.
What the Researchers Studied
The researchers studied Caco-2 cells were exposed to different concentrations of LPS (1 μg/mL-1 mg/mL). The study used a in vitro cell-culture laboratory experiment.
What Effects Did Molecular Hydrogen Have?
This study was aimed to investigate whether H₂ could modulate lipopolysaccharide (LPS)-stimulated dysfunction of the intestinal barrier and whether RhoA-mDia1 signaling is involved. LPS (100 μg/mL) decreased TER and increased fluorescein-isothiocyanate-dextran flux, which were alleviated by H₂-rich medium. Also, H₂ down-regulated LPS-induced oxidative stress. The authors concluded that H₂ improves LPS-induced hyperpermeability of the intestinal barrier and disruptions of TJ and AJ by moderating RhoA-mDia1 signaling.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was Caco-2 cells were exposed to different concentrations of LPS (1 μg/mL-1 mg/mL). The study used a in vitro cell-culture laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base. The original scholarly source is available at https://www.ovid.com/jnls/shockjournal/abstract/10.1097/shk.0000000000000503~hydrogen-rich-medium-ameliorates-lipopolysaccharide-induced?redirectionsource=fulltextview.