Hydrogen Research Study
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Preclinical animal evidenceAnimal studyOther or not reported

Cardioprotective Effect of Hydrogen-rich Saline on Isoproterenol-induced Myocardial Infarction in Rats.

Lei Jing, Yun Wang, Xiao-Min Zhao, Bing Zhao, Ji-Ju Han, Shu-Cun Qin, Xue-Jun Sun · Heart, lung & circulation · 2015

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Independent study record

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H2HUBB Research Library branded molecular hydrogen research image
Primary topic Cardiovascular Health
Evidence type Preclinical animal evidence
Publication type Animal study
Hydrogen method Other or not reported

H2HUBB TAKEAWAY

In male Wistar rats, compared with those in isoproterenol-MI group, hydrogen-rich saline decreased malondialdehyde and 8-hydroxy-desoxyguanosine concentrations, enhanced superoxide dismutase and Na(+)-K(+)-ATPase activity, lowered Ca(2+)-ATPase activity and decreased interleukin-6 and tumour necrosis factor-α levels in the serum and/or cardiac tissue of rats. These results are preclinical and suggest molecular hydrogen's therapeutic potential in the condition studied. Further human research is needed to establish clinical effectiveness.

What the Researchers Studied

Because oxidative stress plays a significant role in the pathophysiology of myocardial infarction (MI), the aim of the study was to investigate whether hydrogen-rich saline has cardioprotective effects against isoproterenol-induced MI in rats.

How Molecular Hydrogen Was Used

Different doses of hydrogen-rich saline (5, 7.5, and 10 mL/kg body weight i.p.) or Vitamin C (250 mg/kg body weight i.g.) were administered to the rats.

What the Researchers Found

Compared with those in isoproterenol-MI group, hydrogen-rich saline decreased malondialdehyde and 8-hydroxy-desoxyguanosine concentrations, enhanced superoxide dismutase and Na(+)-K(+)-ATPase activity, lowered Ca(2+)-ATPase activity and decreased interleukin-6 and tumour necrosis factor-α levels in the serum and/or cardiac tissue of rats. Hydrogen-rich saline pretreatment also diminished infarct size, improved left heart function, and ameliorated pathological changes of the left heart.

H₂ Mechanisms / Biological Findings

From these results, hydrogen-rich saline exerts cardiovascular protective effects against isoproterenol-induced MI at least in part via interactions which evoke antioxidant and anti-inflammatory activities. Infusion with hydrogen gas-saturated saline has recently been reported to exert antioxidant and anti-inflammatory activity that may protect against organ damage induced by oxidative stress. because oxidative stress plays a significant role in the pathophysiology of myocardial infarction (MI), the aim of the study was to investigate whether hydrogen-rich saline has cardioprotective effects against isoproterenol-induced MI in rats.

Authors’ Conclusion

The authors concluded that from these results, hydrogen-rich saline exerts cardiovascular protective effects against isoproterenol-induced MI at least in part via interactions which evoke antioxidant and anti-inflammatory activities.