Hydrogen-Rich Saline Regulates Microglial Phagocytosis and Restores Behavioral Deficits Following Hypoxia-Ischemia Injury in Neonatal Mice via the Akt Pathway.
Hongfei Ke, Dexiang Liu, Tingting Li, Xili Chu, Danqing Xin, Min Han, Shuanglian Wang, Zhen Wang · Drug design, development and therapy · 2020
Research-use notice
Independent study record
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H2HUBB TAKEAWAY
In mice, consistent with the authors' previous report, 10 HI insult significantly increased brain water content (90.73 ± 1.04%) as compared with that observed in the Sham group (87.86 ± 1.31%) [F(3,16)=11.416, p < 0.001; post hoc p <0.001]. HS treatment significantly decreased this increase in brain water content by HI (80.29 ±0.71%, post hoc p <0.01) ( Figure 2A ). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in mice. Consistent with the authors' previous report, 10 HI insult significantly increased brain water content (90.73 ± 1.04%) as compared with that observed in the Sham group (87.86 ± 1.31%) [F(3,16)=11.416, p < 0.001; post hoc p <0.001], while HS treatment significantly decreased this increase in brain water content by HI (80.29 ±0.71%, post hoc p <0.01) ( Figure 2A ).
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment.
What Effects Did Molecular Hydrogen Have?
Reported treatment duration: 30 min. Consistent with the authors' previous report, 10 HI insult significantly increased brain water content (90.73 ± 1.04%) as compared with that observed in the Sham group (87.86 ± 1.31%) [F(3,16)=11.416, p < 0.001; post hoc p <0.001], while HS treatment significantly decreased this increase in brain water content by HI (80.29 ±0.71%, post hoc p <0.01) ( Figure 2A ). The authors concluded that these results demonstrate that HS treatment attenuates neurobehavioral deficits and apoptosis resulting from HI, effects which were associated with reductions in phagocytosis and appear to involve the Akt signaling pathway. 5, 21 Treatment of ischemic injury with hydrogen has been shown to suppress oxidative stress, proinflammatory responses and cellular apoptosis in kidney, brain, liver, heart and intestines.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The reported sample size was 144. The study used a preclinical animal experiment. The reported treatment duration was 30 min.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.