Hydrogen-rich water alleviates neuropathic pain through the modulation of bile acid/Takeda G-protein-coupled receptor 5-mediated autophagy.
Xuejiao Zeng, Jiaqi Lin, Botao Liu, Zehua Su, Yongze Yu, Xin Li, Wenying Duan, Chunzheng Liu, Qianhao Hou, Jinyuan Zhang, Longqiu Yang, Xiaodong Liu, Bifa Fan, Lijun Liao · Pain · 2026
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In mice, sNL induced alterations in the levels of several bile acid metabolites, particularly the secondary bile acids, such as HDCA and DCA, which were significantly decreased but restored by HRW treatment. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in mice. SNL induced alterations in the levels of several bile acid metabolites, particularly the secondary bile acids, such as HDCA and DCA, which were significantly decreased but restored by HRW treatment.
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
The control group for the hydrogen-rich water group received distilled water intervention. SNL induced alterations in the levels of several bile acid metabolites, particularly the secondary bile acids, such as HDCA and DCA, which were significantly decreased but restored by HRW treatment. The authors concluded that sUPPLEMENTAL DIGITAL CONTENT: associated with this article can be found online at http://links.lww.com/PAIN/C400.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment. The hydrogen delivery method was hydrogen-rich water.
Limitations and Safety
Reported limitations: 8 A limitation of this study is the absence of TGR5 overexpression experiments, which would help elucidate whether TGR5 activation directly modulates pain.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.