Hydrogen-rich water attenuates the radiotoxicity induced by tritium exposure in vitro and in vivo.
Hong Li, Yaru Yin, Jing Liu, Binghui Lu, Huimin Wan, Luxun Yang, Weidong Wang, Rong Li · Journal of radiation research · 2021
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Independent study record
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H2HUBB TAKEAWAY
In male BALB/c mice, showed that intragastric administration of HRW effectively promoted the elimination of urinary tritium, decreased the level of serum tritium and tissue-bound tritium (OBT), and attenuated the genetic damage of blood cells in mice exposed to HTO (18.5 MBq/kg). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in male BALB/c mice. Showed that intragastric administration of HRW effectively promoted the elimination of urinary tritium, decreased the level of serum tritium and tissue-bound tritium (OBT), and attenuated the genetic damage of blood cells in mice exposed to HTO (18.5 MBq/kg).
What the Researchers Studied
The researchers studied male BALB/c mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 1 mM H₂ (≈2.02 mg/L); reported treatment duration: 40 min. Showed that intragastric administration of HRW effectively promoted the elimination of urinary tritium, decreased the level of serum tritium and tissue-bound tritium (OBT), and attenuated the genetic damage of blood cells in mice exposed to HTO (18.5 MBq/kg). The authors concluded that in conclusion, HRW is expected to be an effective radioactive elimination agent through the competition effect of isotope exchange or a radioprotective agent by scavenging free radicals induced by HTO exposure. The dotted arrow represents the mechanism of HRW for tritium excretion and treatment. ①The HTO entering the organism is bound to organic molecules to form OBT and EOT. ②The beta particles emitted by OBT, EOT and free HTO induce an increase in intracellular ROS, such as •OH and O₂ •-, through radiolysis of H₂O and oxidation of biomolecules. ③The excess ROS results in DNA oxidative damage and products 8-OHdG. ④The free radicals also lead to membrane lipid peroxidation to destroy the biofilm structure and products MDA. ⑤HRW with a high concentration of hydrogen competitively binds to the binding sites of tritium in organics to facilitate the free or weakly bonded tritium diffusing out of cells through isotope exchange. ⑥The antioxidant activity of HRW can neutralize excess free radicals (•OH, O₂ •- ). ⑦The exhaustion of cellular endogenous antioxidants (GSH and SOD) stimulated by ROS can also be reversed by HRW, resulting in the relief of oxidative stress injury.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was male BALB/c mice. The study used a preclinical animal experiment. The hydrogen delivery method was hydrogen-rich water. The source-reported hydrogen concentration was 1 mM H₂ (≈2.02 mg/L). The reported treatment duration was 40 min.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.