Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro.
Qiang Li, Shinya Kato, Daigo Matsuoka, Hiroshi Tanaka, Nobuhiko Miwa · Medical gas research · 2013
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In 22 patients with PU were recruited in the present study, eLEVATION OF CELL VIABILITY BY PRE-IRRADIATIONAL ADMINISTRATION WITH HYDROGEN-DISSOLVED WATER TO UVA-IRRADIATED HACAT CELLS AS ASSESSED BY MITOCHONDRIAL DEHYDROGENASE-BASED WST-1 ASSAY: In HaCaT cells, the cell viability was obviously increased in the HW-prepared culture medium with UVA-irradiation, comparing with RW-prepared culture medium by WST-1 assay (Figure 11 -d). These findings add human evidence supporting molecular hydrogen's therapeutic potential across the specific human outcomes evaluated in this study.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in 22 patients with PU were recruited in the present study. ELEVATION OF CELL VIABILITY BY PRE-IRRADIATIONAL ADMINISTRATION WITH HYDROGEN-DISSOLVED WATER TO UVA-IRRADIATED HACAT CELLS AS ASSESSED BY MITOCHONDRIAL DEHYDROGENASE-BASED WST-1 ASSAY: In HaCaT cells, the cell viability was obviously increased in the HW-prepared culture medium with UVA-irradiation, comparing with RW-prepared culture medium by WST-1 assay (Figure 11 -d).
What the Researchers Studied
The researchers studied 22 patients with PU were recruited in the present study. The study used a human clinical study. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
PREPARATION FOR HW: HW was prepared with a hydrogen-bubbling apparatus which consists mainly of a water supply section for manufacturing RW with less 0.018 ppm of DH and +184 mV of ORP, and HW with 0.8-1.3 ppm of DH and −602 mV to −583 mV of ORP. It must be emphasized that some stable characteristic indicators and the proprieties for innocuity and harmlessness of hydrogen water were obtained from several separated in vivo and human experiments which the researchers had reported [ 19 – 23 ]. ELEVATION OF CELL VIABILITY BY PRE-IRRADIATIONAL ADMINISTRATION WITH HYDROGEN-DISSOLVED WATER TO UVA-IRRADIATED HACAT CELLS AS ASSESSED BY MITOCHONDRIAL DEHYDROGENASE-BASED WST-1 ASSAY: In HaCaT cells, the cell viability was obviously increased in the HW-prepared culture medium with UVA-irradiation, comparing with RW-prepared culture medium by WST-1 assay (Figure 11 -d). The authors concluded that hW intake via TF was demonstrated, for severely hospitalized elderly patients with PU, to execute wound size reduction and early recovery, which potently ensue from either type-I collagen construction in dermal fibroblasts or the promoted mitochondrial reducing ability and ROS repression in epidermal keratinocytes as shown by immunostain or NBT and WST-1 assays, respectively. Normal human dermal fibroblasts OUMS-36 and normal human epidermis-derived cell line HaCaT keratinocytes were examined in vitro to explore the mechanisms relating to whether hydrogen plays a role in wound-healing at the cellular level. The mechanism means that the minimum volumes of nano size droplets are produced to maximize their surface areas, and so a high-pressure hydrogen gas was strongly incorporated into droplets inside.
Why These Findings Matter
These findings add human evidence supporting molecular hydrogen's therapeutic potential across the specific human outcomes evaluated in this study and contribute to the growing body of molecular-hydrogen research.
How Strong Is This Evidence?
This is human clinical evidence from a human clinical study. H2HUBB interprets the findings in the context of the study design, sample, comparator, and measured outcomes rather than using one publication as a verdict on hydrogen therapy.
Technical Study Details
H2HUBB classifies this publication as human clinical study with human clinical evidence. The research population or model was 22 patients with PU were recruited in the present study. The reported sample size was 12. The study used a human clinical study.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.