Local Treatment of Hydrogen-Rich Saline Promotes Wound Healing In Vivo by Inhibiting Oxidative Stress via Nrf-2/HO-1 Pathway.
Yujie Li, Chengcheng Shen, Xin Zhou, Jianghe Zhang, Xiaoyue Lai, Yiming Zhang · Oxidative medicine and cellular longevity · 2022
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In mice, local treatment of hydrogen-rich water shortened wound closure time and reduced the level of proinflammatory cytokines and lipid peroxidation. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in mice. Local treatment of hydrogen-rich water shortened wound closure time and reduced the level of proinflammatory cytokines and lipid peroxidation.
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
To make hydrogen-rich medium, 10% fetal bovine serum (Hyclone, researchers), 89% MEM (Hyclone, researchers), and 1% penicillin/streptomycin were infused into sterile empty 100 mL saline bag [ 27, 28 ], and then pump the hydrogen into the bag under 0.4 MPa and keep it in 4°C. 48 mice were randomly divided into the control, the local treatment of the saline group (LTS group), the local treatment of the hydrogen-rich water group (LTH group), and the intraperitoneal injection of the hydrogen-rich water group (IIH group). For the IIH group, the mice were intraperitoneally injected with 0.3 mL hydrogen-rich water once a day. Local treatment of hydrogen-rich water shortened wound closure time and reduced the level of proinflammatory cytokines and lipid peroxidation.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment. The hydrogen delivery method was hydrogen-rich water.
Limitations and Safety
Reported limitations: Specific limitation exists in the study.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.