Molecular Hydrogen Ameliorates Anti-Desmoglein 1 Antibody-Induced Pemphigus-Associated Interstitial Lung Disease by Inhibiting Oxidative Stress.
Chang Tang, Lanting Wang, Zihua Chen, Xiangguang Shi, Yahui Chen, Jin Yang, Haiqing Gao, Chenggong Guan, Shan He, Luyao Zhang, Shenyuan Zheng, Fanping Yang, Sheng-An Chen, Li Ma, Zhen Zhang, Ying Zhao, Qingmei Liu, Jiucun Wang, Xiaoqun Luo · International journal of molecular sciences · 2025
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In mice, it was found that the collagen content of the treatment group was comparable to that of the control group and the hydrogen water group at 3 weeks, and was significantly lower than that of the model group ( Figure 5 f,g). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in mice. It was found that the collagen content of the treatment group was comparable to that of the control group and the hydrogen water group at 3 weeks, and was significantly lower than that of the model group ( Figure 5 f,g).
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
HW (1.6 ppm H₂; Shanghai Yiqingquan Health Technology Co., Ltd., Shanghai, China) was stored in aluminum-lined bags and replaced daily to maintain its concentration, which was verified by gas chromatography (GC-2014, Shimadzu, Kyoto, Japan). The mice in the control group (Control) and the model group (P-ILD) were fed with normal water, and the mice in the hydrogen water group (H₂ ) and the treatment group (P-ILD + H₂ ) were fed with hydrogen water. During the feeding process, the aluminum foil bag containing hydrogen water was directly connected to the automatic water dispenser, and the hydrogen water was replaced once a day to ensure that the mice could drink hydrogen water containing sufficient hydrogen molecular concentrations at any time ( Figure 8 ). It was found that the collagen content of the treatment group was comparable to that of the control group and the hydrogen water group at 3 weeks, and was significantly lower than that of the model group ( Figure 5 f,g). Consistently, the findings indicated that the sustained consumption of hydrogen water in mice with P-ILD effectively mitigated oxidative stress, leading to a reduction in pulmonary interstitial inflammation and the prevention of progression to pulmonary fibrosis.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment. The hydrogen delivery method was hydrogen-rich water.
Limitations and Safety
Safety information: Further studies are warranted to explore the clinical applicability and safety of H₂ therapy in P-ILD and to elucidate its underlying molecular mechanisms.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.