[Protective effects of hydrogen-rich medium on lipopolysaccharides-induced injury in human periodontal ligament cells].
Min Zhou, Zuo-Lin Wang · Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2018
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In a laboratory model, cCK-8 results showed that hydrogen could significantly improve hPDLCs growth and decrease cellular apoptosis under LPS stimulation (P<0.05). The authors concluded that the hydrogen-rich medium can effectively improve hPDLCs proliferation activity and antioxidant capacity and reduce apoptosis and oxidative stress under LPS stimulation. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen was evaluated in this publication and summarizes the source-grounded findings below. CCK-8 results showed that hydrogen could significantly improve hPDLCs growth and decrease cellular apoptosis under LPS stimulation (P<0.05).
What the Researchers Studied
The study used a laboratory experiment.
What Effects Did Molecular Hydrogen Have?
HPDLCs were isolated, and then cultured with normal medium+1 μg·mL−1 LPS or with hydrogen-rich medium+ 1 μg·mL−1 LPS. Cell proliferation activity was assessed using a cell counting kit-8 (CCK-8), and lactic dehydrogenase (LDH) release was also detected. CCK-8 results showed that hydrogen could significantly improve hPDLCs growth and decrease cellular apoptosis under LPS stimulation (P<0.05). The authors concluded that the hydrogen-rich medium can effectively improve hPDLCs proliferation activity and antioxidant capacity and reduce apoptosis and oxidative stress under LPS stimulation. In this study, lipopolysaccharides (LPS) was used to damage human periodontal ligament cells (hPDLCs) and consequently investigate the protective effects of hydrogen on reducing oxidative stress and cellular apoptosis rate.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The study used a laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.