Organ preservation solution containing dissolved hydrogen gas from a hydrogen-absorbing alloy canister improves function of transplanted ischemic kidneys in miniature pigs.
Eiji Kobayashi, Motoaki Sano · PloS one · 2019
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In an animal model, after storage of the kidney in hydrogen-free preservation solution for 1 hour before transplantation, no urine production was observed and blood flow was not detected in the transplanted kidney at sacrifice on postoperative day 6. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how hydrogen gas infusion into the tissue-bath preparation was evaluated in this publication and summarizes the source-grounded findings below. After storage of the kidney in hydrogen-free preservation solution for 1 hour before transplantation, no urine production was observed and blood flow was not detected in the transplanted kidney at sacrifice on postoperative day 6.
What the Researchers Studied
The study used a preclinical animal experiment.
What Effects Did Molecular Hydrogen Have?
The device for transferring hydrogen was assembled just before use and hydrogen was infused into the organ preservation solution from the hydrogen-absorbing alloy canister via a pressure regulating valve ( Fig 1B and 1C ). 10.1371/journal.pone.0222863.g001 Fig 1 Novel method of dissolving hydrogen gas in organ preservation solution by using a hydrogen-absorbing alloy canister. (A) Mechanism of the hydrogen absorbing alloy. (B) Process of dissolving hydrogen in the organ preservation solution by using the hydrogen-absorbing alloy canister. (1) A hydrogen-absorbing alloy canister and a pressure regulating valve are connected to a bag of organ preservation solution. (2) Hydrogen gas is injected through the rubber stopper using a bottle needle. After flushing with either hydrogen-containing cold ETK solution or hydrogen-free cold ETK solution for 5 minutes, the harvested kidneys were immersed and stored in the same ETK solution for 1 to 4 hours until transplantation into the recipient. After storage of the kidney in hydrogen-free preservation solution for 1 hour before transplantation, no urine production was observed and blood flow was not detected in the transplanted kidney at sacrifice on postoperative day 6. The authors concluded that in conclusion, the researchers developed a device that can be used to rapidly infuse hydrogen gas into organ preservation solution at the site of donor organ removal.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The study used a preclinical animal experiment. The hydrogen delivery method was hydrogen gas infusion into the tissue-bath preparation.
Limitations and Safety
Reported limitations: While it has been shown that hydrogen gas is effective for preventing ischemia reperfusion injury [ 1 – 4 ], hydrogen gas is flammable at high concentrations and clinical use is limited by safety considerations.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.