Treatment with Hydrogen-Rich Water Improves the Nociceptive and Anxio-Depressive-like Behaviors Associated with Chronic Inflammatory Pain in Mice.
Santiago Coral-Pérez, Ignacio Martínez-Martel, Maria Martínez-Serrat, Gerard Batallé, Xue Bai, Christie R A Leite-Panissi, Olga Pol · Antioxidants (Basel, Switzerland) · 2022
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In mice, treatment with HRW intraperitoneally administered at 2T per day over two consecutive days normalized the increased immobility time observed in CFA-injected animals treated with VEH in the TST ( p < 0.0001; one-way ANOVA and Student–Newman–Keuls test) and FST ( p < 0.0001; one-way ANOVA and Student–Newman–Keuls test). These preclinical findings add evidence about the molecular-hydrogen effects, outcomes, and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in mice. Treatment with HRW intraperitoneally administered at 2T per day over two consecutive days normalized the increased immobility time observed in CFA-injected animals treated with VEH in the TST ( p < 0.0001; one-way ANOVA and Student–Newman–Keuls test) and FST ( p < 0.0001; one-way ANOVA and Student–Newman–Keuls test).
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 0.3 mM H₂ (≈0.605 mg/L). Treatment with HRW intraperitoneally administered at 2T per day over two consecutive days normalized the increased immobility time observed in CFA-injected animals treated with VEH in the TST ( p < 0.0001; one-way ANOVA and Student–Newman–Keuls test) and FST ( p < 0.0001; one-way ANOVA and Student–Newman–Keuls test). The authors concluded that the intraperitoneal administration of HRW, besides reducing inflammatory pain, also inhibited the depressive- and anxiolytic-like behaviors associated and the over expression of 4-HNE, p-IKBα, and BAX in paws and amygdala. Using male mice with chronic inflammatory pain incited by the subplantar injection of complete Freud's adjuvant (CFA), the researchers assessed the actions of hydrogen-rich water (HRW) systemically administered on: (1) the nociceptive responses and affective disorders associated and (2) the oxidative (4-hydroxy-2-nonenal; 4-HNE), inflammatory (phosphorylated-NF-kB inhibitor alpha; p-IKBα), and apoptotic (Bcl-2-like protein 4; BAX) changes provoked by CFA in the paws and amygdala. These outcomes were corroborated by other studies performed with H₂ [ 54 ] and with other compounds such as hydrogen sulfide donors and several antioxidants, which also activated the Nrf2/HO-1 and/or NQO1 pathway for alleviating chronic inflammatory, osteoarthritis, and neuropathic pain in rodents [ 5, 21, 45, 55 ].
Why These Findings Matter
This publication adds useful evidence about where molecular hydrogen did and did not change the measured outcomes, helping define the larger therapeutic evidence base.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment. The hydrogen delivery method was hydrogen-rich water. The source-reported hydrogen concentration was 0.3 mM H₂ (≈0.605 mg/L).
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.